![]() This indicated that E2 intradiscal injection may modulate IVD extracellular matrix (ECM) production towards anabolism however, it is not known whether E2-induced changes at the gene expression level can be translated into an increase in IVD matrix production. ![]() In ovariectomised female rats with IVDD, a 28-day treatment with E2 downregulated matrix metalloproteinase ( MMP) 3 and MMP13 expression and up-regulated type II collagen ( COL2A1) expression. In two-dimensional (2D) cultures, 17β-oestradiol (E2) was shown to enhance human IVD cell proliferation, and to exert anti-apoptotic effects in human cartilaginous endplate cells and rat nucleus pulposus (NP). ![]() Several studies demonstrated positive effects of oestrogen-related signalling on IVD metabolism. This indicates possible benefits of oestrogen therapy for the IVDs of postmenopausal women undergoing LMHFV exercises. The combination of E2 + LMHFV induced a protective effect against cell loss and decreased IL-6 production compared to the LMHFV group. Interestingly, LMHFV alone induced cell loss and increased IL-6 production compared to the control. Minor changes in gene expression, cellularity and matrix metabolism were observed after E2 treatment, except for a significant increase in matrix metalloproteinase (MMP)-3 and interleukin (IL)-6 production. Bovine IVDs ( n = 6 IVDs/group) were treated with either (i) E2, (ii) LMHFV or (iii) the combination of E2 + LMHFV for 2 or 14 days. The present study investigated the effect of 17β-oestradiol (E2) and LMHFV in an IVD organ culture model. Low-magnitude, high-frequency vibration (LMHFV) is applied as a therapeutic approach for postmenopausal osteoporosis however, less is known regarding possible effects on the intervertebral disc (IVD) and whether these may be oestrogen-dependent. Postmenopausal women are at an increased risk for intervertebral disc degeneration, possibly due to the decrease in oestrogen levels.
0 Comments
Leave a Reply. |